Table 2.

Apparent dissociation constants for the YadA type I collagen binding

Mutation	RUmaxa	KD(app) (μM)	% of wt
wt	3343±160	0.28±0.06	100
V98D, N99A	356±37	7.34±2.72	4
K108A, L110A	3378±134	2.84±0.48	11
Q124A, K125A	1619±64	0.98±0.16	30
H159A, H162A	916±27	2.49±0.32	12
K148A, D150A	1588±74	2.0±0.21	15
D180A, E182A	2209±154	25.7±3.7	1
E190A, S191A	3371±143	0.59±0.08	50
E80A, K83A	2612±96	1.51±0.26	20
N166A, Y169A	4599±731	1.77±0.64	17
R133A	1208±17	2.9±0.08	10
a The RUmax appeared to vary depending on the age of the chip and how efficiently it was cleaned after each run.