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. 2004 Feb 12;23(4):844–856. doi: 10.1038/sj.emboj.7600101

Figure 1.

Figure 1

BMP4 synexpression group. (A) Simplified scheme of the BMP signaling pathway with coexpressed components (synexpression group member genes) indicated in orange. (B) Whole-mount in situ hybridization for bmp4, vent2, bambi and smad7 in Xenopus tailbud embryos reveals their highly similar expression pattern in the dorsal eye (de), heart region (he) and proctodeum (pr). (C) RT–PCR analysis of ectodermal explants (animal caps) from embryos injected animally with 2 ng bmp4 RNA and either treated or untreated with cycloheximide (CHX) from stage 8 onwards. Animal caps were collected at stage 9 (late blastula) or 10 (early gastrula) and analyzed for expression of the indicated RNAs. The samples were normalized by ef1α and histone h4 expression. (D) RT–PCR analysis of animal caps from embryos injected animally with 2 ng vent1, vent2 or xhox3 RNA and treated with CHX from stage 8 for 3 h.