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. 2004 Feb 12;23(4):844–856. doi: 10.1038/sj.emboj.7600101

Figure 5.

Figure 5

Transgenic analysis of the vent2 promoter. (A) Scheme of the vent2 promoter (Hata et al, 2000; Henningfeld et al, 2000, 2002). S1, Smad1 motif (GCAT); OAZ, binding site (TGGAGC) for the Smad cofactor OAZ; Lef, putative Lef/TCF motif (CCTTTGAT); V2, Vent2 motifs. (B) In situ hybridization of vent2 at the tailbud stage. (C–J) gfp in situ hybridization of tailbud stage embryos transgenic for the indicated constructs (see panel A). BREx4-Xcol, four copies of the vent2 BRE in front of the collagen X minimal promoter. The percentage of embryos with the depicted expression pattern is shown.