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. 2004 Feb 12;23(4):919–929. doi: 10.1038/sj.emboj.7600103

Figure 2.

Figure 2

Cdc25p remains nuclear in clp1Δ anaphase B cells. (A) Compression of image stacks of cdc25-GFP (KGY 4337) and cdc25-GFP clp1Δ (KGY 4341) asynchronous cells grown at 25°C in YE medium. Tail-less arrows (∨) indicate binucleate cells without Cdc25p-GFP nuclear fluorescence and arrows (↓) indicate binucleate cells with nuclear Cdc25p-GFP. Scale bar indicates 10 μm. (B) Percentage of anaphase B cells with nuclear Cdc25p-GFP from cdc25-GFP sid4-GFP (KGY 877) and cdc25-GFP sid4-GFP clp1Δ (KGY 148) asynchronous cells grown at 25°C in YE medium. Anaphase B cells were those with two well-separated spindle pole bodies (Sid4p-GFP) and no septum.