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. 2004 Feb 5;23(4):908–918. doi: 10.1038/sj.emboj.7600105

Figure 2.

Figure 2

dis2Δ cells have a prolonged G2 DNA damage checkpoint. (A) Serial dilutions of the indicated strains were spotted onto YES agar containing no drug, 0.01% MMS or 62 ng/ml 4-NQO. (B) DAPI-stained cells of strains grown in the presence of 0.008% MMS for 16 h. (C) Wild type (○), dis2Δ (•), chk1Δ (□) and rad3-136 (▵) strains were treated with the indicated doses of UV-C and ionising radiation, and per cent survival compared with unirradiated controls was determined. (D) Synchronous G2-phase cultures of wild-type and dis2Δ strains, obtained by centrifugal elutriation, were treated with 150 J/m2 UV-C radiation (○) or left untreated (•), and the per cent of cells passing mitosis was followed over time.