. 2013 Oct 2;35(5):465–474. doi: 10.1155/2013/290670

Table 2.

Typical IL-13 calibration curve data obtained during assay validation.

IL-13 standard concentration (pg/mL)	Wells	OD	OD mean	CV (%)	BackCalc mean	BackCalc Cv in %	Accuracy (%)	Accuracy mean (%)
40	A11	3.038	3.073	1.6	39.6	2.86	97.05	99.05
40	A12	3.108	3.073	1.6	39.6	2.86	101.06	99.05
30	B11	2.543	2.592	2.6	30.2	3.9	97.78	100.56
30	B12	2.64	2.592	2.6	30.2	3.9	103.33	100.56
20	C11	1.897	1.926	2.1	20.2	2.65	99.27	101.16
20	C12	1.955	1.926	2.1	20.2	2.65	103.06	101.16
10	D11	1.024	1.058	4.5	10.1	5.04	97.07	100.66
10	D12	1.091	1.058	4.5	10.1	5.04	104.25	100.66
5	E11	0.556	0.559	0.5	4.9	0.62	97.48	97.91
5	E12	0.561	0.559	0.5	4.9	0.62	98.34	97.91
2.5	F11	0.333	0.328	1.8	2.5	2.46	102.45	100.7
2.5	F12	0.324	0.328	1.8	2.5	2.46	98.94	100.7
1.25	G11	0.21	0.213	1.5	1.3	2.93	98.51	100.6
1.25	G12	0.215	0.213	1.5	1.3	2.93	102.68	100.6
0.63	H11	0.161	0.159	1.6	0.6	4.81	104.68	101.23
0.63	H12	0.158	0.159	1.6	0.6	4.81	97.79	101.23
0.31	D9	0.135	0.137	2.2	0.3	11.35	102.43	111.37
0.31	D10	0.139	0.137	2.2	0.3	11.35	120.31	111.37
0.21	E9	0.124	0.125	0.7	0.2	6.75	83.33	87.51
0.21	E10	0.126	0.125	0.7	0.2	6.75	91.69	87.51
0.16	F9	0.123	0.123	0.5	0.1	5.82	96.95	93.12
0.16	F10	0.122	0.123	0.5	0.1	5.82	89.29	93.12