Table 3.

Parallelism assessment of IL-13 assay in human plasma under acidic conditions. The accuracy was calculated using the IL-13 concentration determined at dilution 1 : 2 as a reference value. Parallelism was considered acceptable when accuracy was between 70 and 130% for at least 5/6 samples at each dilution.

Dilution	Raw result (pg/mL)		Accuracy (O/E, %)
	Observed (O)	Expected (E)
Sample 1
1 : 2	0.425
1 : 3	0.250	0.283	88.2
1 : 4	0.195	0.213	91.8
1 : 6	N.C	N.C.	N.C.
Sample 2
1 : 2	1.280
1 : 3	0.760	0.853	89.1
1 : 4	0.525	0.640	82.0
1 : 6	0.362	0.427	84.8
Sample 3
1 : 2	0.745
1 : 3	0.517	0.497	104.0
1 : 4	0.425	0.373	114.1
1 : 6	0.285	0.248	114.8
Sample 4
1 : 2	1.190
1 : 3	0.763	0.793	96.2
1 : 4	0.540	0.595	90.8
1 : 6	0.365	0.397	92.0
Sample 5
1 : 2	1.330
1 : 3	1.020	0.887	115.0
1 : 4	0.830	0.665	124.8
1 : 6	0.553	0.443	124.8
Sample 6
1 : 2	0.270
1 : 3	0.207	0.180	114.8
1 : 4	0.148	0.135	109.3
1 : 6	N.C	N.C.	N.C.

N.C.: value not calculated as being outside the calibration range.