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. 2013 Oct 28;8(10):e77791. doi: 10.1371/journal.pone.0077791

Table 2. Primers used for cloning.

Primer Sequence Cloning site Source Target Resulting Vector
SP/MMI1 P1 CGGGATCCATGATTATTTACAAGGATATC BamHI pUG35-MMI1 pUG35 pUG35- (N)MMI1
ASP/MMI1 P1 CGGAATTCAGCGGTTTGTTGTAGACG EcoRI pUG35-MMI1 pUG35 pUG35- (N)MMI1
SP/MMI1 P2 CGGGATCCATGTTCCGTCTACAACAAACCGCTTTTG BamHI pUG35-MMI1 pUG35 pUG35- (V)MMI1
ASP/MMI1 P2 CGGAATTCTGGGTCCATGGATTCACC EcoRI pUG35-MMI1 pUG35 pUG35- (V)MMI1
SP/MMI1 P3 CGGGATCCATGGAGTTCTTCACTGGTGAA BamHI pUG35-MMI1 pUG35 pUG35-(C)MMI1
ASP/MMI1 P3 GGAATTCGATCTTTTCTTCCACA EcoRI pUG35-MMI1 pUG35 pUG35-(C)MMI1
SP/MMI1 P1 CGGGATCCATGATTATTTACAAGGATATC BamHI pUG35-MMI1 pUG35 pUG35- (N+V)MMI1
ASP/MMI1 P2 CGGAATTCTGGGTCCATGGATTCACC EcoRI pUG35-MMI1 pUG35 pUG35- (N+V)MMI1