Table 2. Primers used for cloning.
Primer | Sequence | Cloning site | Source | Target | Resulting Vector |
SP/MMI1 P1 | CGGGATCCATGATTATTTACAAGGATATC | BamHI | pUG35-MMI1 | pUG35 | pUG35- (N)MMI1 |
ASP/MMI1 P1 | CGGAATTCAGCGGTTTGTTGTAGACG | EcoRI | pUG35-MMI1 | pUG35 | pUG35- (N)MMI1 |
SP/MMI1 P2 | CGGGATCCATGTTCCGTCTACAACAAACCGCTTTTG | BamHI | pUG35-MMI1 | pUG35 | pUG35- (V)MMI1 |
ASP/MMI1 P2 | CGGAATTCTGGGTCCATGGATTCACC | EcoRI | pUG35-MMI1 | pUG35 | pUG35- (V)MMI1 |
SP/MMI1 P3 | CGGGATCCATGGAGTTCTTCACTGGTGAA | BamHI | pUG35-MMI1 | pUG35 | pUG35-(C)MMI1 |
ASP/MMI1 P3 | GGAATTCGATCTTTTCTTCCACA | EcoRI | pUG35-MMI1 | pUG35 | pUG35-(C)MMI1 |
SP/MMI1 P1 | CGGGATCCATGATTATTTACAAGGATATC | BamHI | pUG35-MMI1 | pUG35 | pUG35- (N+V)MMI1 |
ASP/MMI1 P2 | CGGAATTCTGGGTCCATGGATTCACC | EcoRI | pUG35-MMI1 | pUG35 | pUG35- (N+V)MMI1 |