Figure 2. Tc Muc inhibits anti-CD3 restimulation of activated CD4⁺ T cells.

Naϊve splenic CD4⁺ T cells were stimulated in 48-well culture plates coated with anti-CD3 (5 µg/mL) in the presence or absence of Tc Muc (20 µg/mL) or the same amount of the bovine mucin as control. After 72 hr of stimulation, activated CD4⁺ T cells were harvested and restimulated for an additional 3 days with plate-bound anti-CD3 in the presence or absence of the TcMuc (20 µg/mL). Proliferation was measured 72 h after stimulation by [³H]thymidine incorporation. *Differences between Tc mucin treatment versus anti-CD3 stimulated positive control are significant (P≤0.05). The data represented above are representative of one of three experiments with similar results.