Table 1.
Labelling statistics
| yTAFs labelled | No. of images |
Lobe A |
Lobe B |
Lobe C |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| n1 | n2 | Sector 1 | Sector 2 | Sector 3 | Sector 4 | Sector 5 | Sector 6 | Sector 7 | Sector 8 | Sector 9 | |
| TBP | 1464 | 607 | 3.2 | 0.9 | 0.8 | 1.3 | 0.8 | 1.2 | 0.8 | 0.8 | 0.9 |
| HA-TBP | 908 | 599 | 1.1 | 5.9 | 0.3 | 1 | 0.5 | 1.5 | 0.6 | 6.7 | 8.1 |
| Ab1-TAF1 | 1144 | 420 | 6.0 | 15.1 | 8.8 | 0.5 | 0 | 0.9 | 1.4 | 0.9 | 4.9 |
| Ab2-TAF1 | 1180 | 590 | 0.4 | 0.2 | 0.7 | 1.3 | 2 | 0.7 | 0.3 | 3.4 | 0.2 |
| Ab3-TAF1 | 286 | 126 | 0.4 | 0.4 | 0.7 | 0.6 | 2 | 0.9 | 0.5 | 5.1 | 0.9 |
| Ab4-TAF1 | 729 | 422 | 0.5 | 1.8 | 5.2 | 0.2 | 0.7 | -0.6 | -0.3 | -0.8 | -1.3 |
| N-term TAF5 | 888 | 248 | -0.8 | -1 | 0.8 | -0.8 | 1 | 0.3 | 5.6 | -0.8 | 1.3 |
| C-term TAF5 | 639 | 202 | 0.2 | 3.6 | 3.9 | 1.7 | 3.4 | 0.9 | 0.2 | 0.7 | 0.7 |
| TAF7 |
750 |
164 |
0.1 |
1.0 |
5.7 |
0.4 |
1.3 |
1.3 |
0.4 |
1.0 |
1.0 |
| For each antibody used to detect the position of TBP or yTAFs, the number of recorded images (n1) and the number of trilobed views (n2) are indicated. Each lobe was divided into three sectors as shown in Figure 1B. The values reported for each sector represent deviations from the average binding frequency (in sigma folds). Bold characters represent values higher than 3 sigmas. | |||||||||||