Figure 3. TGRL and DHA do not affect basal or TNFα-induced NF-κB and AP-1 activity.
(A) Active NF-κB (phospho-p65) measured by ELISA in nuclear extract of HAEC treated for 30 min with DHA (10µM) or TGRL (10 mg/dl ApoB) alone or simultaneously with TNFα (0.3ng/ml). n=3–8. (B) Phosphorylated c-Jun expression measured by ELISA in nuclear extract of HAEC treated as in (A). n=3–9. *p<0.05; **p<0.01; ***p<0.001 vs. non-stimulated.