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. Author manuscript; available in PMC: 2013 Oct 28.
Published in final edited form as: Circ Res. 2012 Aug 8;111(8):10.1161/CIRCRESAHA.112.270314. doi: 10.1161/CIRCRESAHA.112.270314

Figure 7. Monocyte adhesion to activated HAEC correlates with changes in VCAM-1 expression.

Figure 7

HAEC were treated with pro- or anti-atherogenic TGRL plus 0.3ng/mL TNFα, or transfected with IRF-1 siRNA or cDNA construct, or miR-126 inhibitor prior to TNFα stimulation. Monocyte adhesion was performed as described in Methods and plotted relative to the number arrested in response to 0.3ng/mL TNFα plus control transfection. Black dots represented 0.1ng/ml (−27%), 0.3ng/ml (0%), or 1.0ng/ml (25%) ng/ml TNFα, respectively. Ellipse centroid represents the mean value, the width and height correspond to the SEM of VCAM-1 expression or monocyte adhesion, respectively. (n=3–5 individual experiments for each treatment.)