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. 2013 Oct 28;8(10):e78979. doi: 10.1371/journal.pone.0078979

Figure 4. The eIF4B requirement for Lamγ2 and MYC protein expression demonstrated by shRNA knockdown.

Figure 4

A) Effect of eIF4B knockdown on eIF4B, Lamγ2, and MYC mRNA levels. RNA from POE9n and SCC-68 cells transduced to express shRNAs targeting eIF4B or Luciferase was analyzed by qPCR for eIF4B, Lamγ2, and MYC transcripts. Results of each cell line was internally normalized to GAPDH mRNA levels and expressed relative to that of cells transduced to express shLuciferase, set at an arbitrary value of 1. B) Western blot analysis of the time-course of eIF4B protein reduction in SCC cells transduced to express shRNA.eIF4B#1 and examined 1, 2, and 3 d after transduction. C) Western blot analysis of the premalignant cell line (POE9n) and two SCC lines (SCC-13, SCC-68) transduced with three shRNA vectors targeting EIF4B and a control shRNA vector targeting luciferase.