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. 2013 Oct 28;8(10):e76756. doi: 10.1371/journal.pone.0076756

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© 2013 Gritti et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

The simulated trajectories were obtained by assuming and were rebinned to reproduce the experimental time acquisition step (25′), as detailed in File S1 (paragraph S4:“Analysis of dendritic and NK cell interactions”). The NK cell confinement ratio (C_R), the 3D speed (v_3D) and the trajectory length (L_traj) computed on more than 200 trajectories (NK cells) are reported in Panels A, B (confinement ratio), C, D (speed) and E, F (trajectory length). In each panel the labels “stimulated” and “un-stimulated” indicate the cases under stimulus (lipopolysaccharide injected i.v. in the mouse for the experimental data and for the simulated data) and under no stimulus (no lipopolysaccharide injected i.v. in the mouse for the experimental data and for the simulated data). Interacting and non-interacting NK cells were determined in the experimental and simulated data by the instantaneous NK cell velocity and the NK – dendritic cell distance, as described in the text. The horizontal bars indicate on each data set the average value.

Inline graphic — The simulated trajectories were obtained by assuming and were rebinned to reproduce the experimental time acquisition step (25′), as detailed in File S1 (paragraph S4:“Analysis of dendritic and NK cell interactions”). The NK cell confinement ratio (C_R), the 3D speed (v_3D) and the trajectory length (L_traj) computed on more than 200 trajectories (NK cells) are reported in Panels A, B (confinement ratio), C, D (speed) and E, F (trajectory length). In each panel the labels “stimulated” and “un-stimulated” indicate the cases under stimulus (lipopolysaccharide injected i.v. in the mouse for the experimental data and for the simulated data) and under no stimulus (no lipopolysaccharide injected i.v. in the mouse for the experimental data and for the simulated data). Interacting and non-interacting NK cells were determined in the experimental and simulated data by the instantaneous NK cell velocity and the NK – dendritic cell distance, as described in the text. The horizontal bars indicate on each data set the average value.