Cell viability as measured via MTT assay with HepG2 cells transfected with polyplexes formed at N/P ratios of 2, 5, 10, 15, 20, and 30 with pDNA and all the P(MAG-b-methacrylate) containing the charge blocks of PMAEMT, PDMAEMT, and PTMAEMT polymers in (a) serum free Opti-MEM and (b) DMEM with 10% serum. Error bars are representative of the standard deviation of analyzed data from three replicates. All measurements found to be statistically significant (p<0.05) as compared to cells only are marked with an asterisk.