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. Author manuscript; available in PMC: 2013 Oct 28.
Published in final edited form as: Nat Methods. 2011 Sep 11;8(10):871–878. doi: 10.1038/nmeth.1694

Figure 2. Cerebellar microcirculatory dynamics in freely behaving mice.

Figure 2

a. Microvasculature in cerebellar cortex of a freely behaving mouse, after intravascular injection of fluorescein-dextran dye. The image shown (300 × 300 pixels; 440 μW illumination power at the specimen plane) is the standard deviation of a 10 s movie, a computation that highlights vasculature (Online Methods). Colored dots mark the locations of corresponding measurements in (c, d). Scale bar is 50 μm.

b. Map of erythrocyte flow speeds averaged over an interval of 30 s for vessels of (a). Scale bar is 50 μm.

c, d. Erythrocyte flow speeds, (c), and vessel diameter changes, (d), for the 4 vessels marked in (a). Blue shading marks periods of movement about the cage. Red shading indicates running on an exercise wheel. White shading marks when the mouse rested orbarely moved. Black vertical lines separate different records from the same mouse and specimen field.