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. Author manuscript; available in PMC: 2014 Sep 23.
Published in final edited form as: Vaccine. 2013 Aug 1;31(41):10.1016/j.vaccine.2013.07.030. doi: 10.1016/j.vaccine.2013.07.030

Figure 5. PPS-selected B cells phenotypically resemble human B-1 cells.

Figure 5

Four healthy human donors were immunized with PPV. Lymphocyte enriched peripheral blood samples were stained with immunofluorescent PPS and antibodies to be evaluated by flow cytometry on day-7. Representative plot of CD3 expression on CD19+ B cells (A). Representative plot of CD20 expression on CD19+ B cells (B). Representative plot of PPS23F-selected IgM+ memory cells gated for CD43+ and CD5+ expression and plotted for CD43 and CD70 expression (C). All flow cytometry results were analyzed and plotted using Fluorescence Minus One controls (FMO). 100,000 events were recorded.