Figure 4. Myc-NMD3 nucleolar localization is independent of CRM1. (A and B) Cells were depleted of RPA194 using RNAi, transfected with (A) Myc-NMD3 or (B) Myc-NMD3-NES expression vectors and treated with LMB as shown for 4 h. Cells were fixed and stained for Myc-NMD3 using Myc-epitope tag antibody. Scale bar, 10 µm. (C) Quantification of nucleolar expression of Myc-tagged NMD3 and Myc-NMD3-NES. The percentage of cells with nucleolar expression is shown. Data represents means ± SD, n = 2 experiments.