Figure 5. CRM1 nucleolar accumulation is dependent on NMD3. (A) Cells were transfected with control or NMD3 targeting siRNAs and incubated for 48 h, followed by Western analysis for NMD3 and CRM1. (B) Quantitative PCR for the expression of NMD3 transcript. qPCR was normalized to GAPDH. (C) Cells were silenced for NMD3 as in A and treated with ActD (50 ng/ml) for 3 h and stained for CRM1. Scale bars, 10 µm. (D) Quantification of cells with CRM1 nucleolar accumulation in (C). Data represents means ± SD, n = 2 experiments. **, p < 0.001.