Table I.
List of primers used for polymerase chain reaction amplification of the IRS-1 gene.
| Forward primer (5′→3′) | Reverse primer (5′→3′) | TA (°C) | ||
|---|---|---|---|---|
| 1S′-990: | TCTGCTCAGCGTTGGTGGT | 1A-1815: | GCGGAACTCATCACTCATG | 59 |
| 2S-1732: | ATGCAGGTGGATGACTCTG | 2A-2686: | GCATCATCTCTGTGTACTCCTC | 57 |
| 3S-2606: | GCACATCCCCTACCATTACC | 3A-3330: | GGATCTTGGCAATGAGTAGTAGG | 57 |
| BS-3217: | ATGAACATGTCACCAGTGGG | BA-3838: | CCTCAGTGCCAGTCTCTTCC | 58 |
| BS3-3776: | CTTCTGTCAGGTGTCCATCC | 4A3-4845: | CAGAGGCGAAGAACAGAATTC | 59 |
| 1S′-990: | TCTGCTCAGCGTTGGTGGT | 1A22-1557: | GACGTTCTTTGTCTGACCCAG | 60 |
| 1S22-1491: | ACCCGCATTCAAAGAGGTC | 1A-1815: | GCGGAACTCATCACTCATG | 60 |
| 2S-1732: | ATGCAGGTGGATGACTCTG | 2A2-2140: | AGCGGCTGTGGTTGAG | 58 |
| 2S2-2071: | ACCAACAGAACCCACGC | 2A-2686: | GCATCATCTCTGTGTACTCCTC | 58 |
| 3S-2606: | GCACATCCCCTACCATTACC | 3A2-2950: | GTGGGGCAGATACGCTC | 59 |
| 3S2-2892: | TGGCCGAAAGGGCAGT | 3A-3330: | GGATCTTGGCAATGAGTAGTAGG | 59 |
| BS-3217: | ATGAACATGTCACCAGTGGG | BA2-3565: | CAGCTGTGTCCACTTCTCG | 60 |
| BS2-3516: | CCACCATCAGGTTCTGCAG | BA-3838: | CCTCAGTGCCAGTCTCTTCC | 60 |
| BS3-3776: | CTTCTGTCAGGTGTCCATCC | 4A-4191: | CGAGTGGGCAGCCAGCT | 60 |
| 4S-4019: | GCTACGTGGACACCTCG | 4A2-4461: | CTCAAAGGAAGCAGAGCTG | 56 |
| 4S2-4425: | CGAGGATGTGAAACGCC | 4A3-4845: | CAGAGGCGAAGAACAGAATTC | 60 |
IRS, insulin receptor substrate.