Table II.

List of primers used for polymerase chain reaction amplification of the IRS-2 gene.

Forward primer (5′→3′)		Reverse primer (5′→3′)		TA (°C)
ATG 5S:	GCGCAAGGGTGGGAGGGAGC	A2:	CTCAGGGGGCTCCCAGCCA	68
B1:	CTGGAGGCCATGAAGGCGCTC	R:	GGCGAAGGCACTACAGGGTG	67
S:	AGGAGGAGCGTCTGGAGCCTC	T:	GTAGTCGGAGAGCGGAGACC	67
U:	TCGCTCTTGTCCGCCAGCAG	V3:	CATCTCGGTGTAGTCACCATTG	68
Z:	CCTCATCGTTGTCCTCGGAC	XX2:	AGTGGTGGGACAAGAAGTCA	62
K2:	CTTCCAGAATGGTCTCAACTAC	EX1:	GGCTTCTGGGTCAAGGT	62
EX2:	TGACCCAGGTCCTAGCTG	XX:	TGACATGTGCACATCCTGGTG	58
ATG 5S:	GCGCAAGGGTGGGAGGGAGC	BATG:	AGCCCTCCTGCTCCTGCTCG	66
C:	TCTACACCAAGGACGAGTACTTCG	D:	GATGTTCATGAGCTGCAGC	60
18UP:	GCTTCGTGAAGCTCAACTGCGAG	19DW:	CGACGATTGGCTCTTACTGCG	66
B1:	CTGGAGGCCATGAAGGCGCTC	A2:	CTCAGGGGGCTCCCAGCCA	71
G1:	AAGTGCAGCTCGTGCAGGG	cM:	AGGTCCTCTTGCGCAGCCCTC	69
CC:	TGGACGAGTACGGCTCCAG	N:	CCCTGGGCTGCAAGATCTGCTT	60
O:	GCAGGAGCGACGACTACATG	P:	GCCATCTGCATGCTCCATGG	60
Q:	GAGGACAGTGGGTACATGCG	R:	GGCGAAGGCACTACAGGGTG	60
Z:	CCTCATCGTTGTCCTCGGAC	W:	CGCTGCTTTTCCTGAGAGAGAC	62
X:	ACCCCAAGCGCCACAACTCGG	Y:	CTTGTCTCCCGGCTGAGGAAG	64

IRS, insulin receptor substrate.