Figure 6. CD36 contributes to IFN-γ production by T cells stimulated with IRBC-treated DCs.

FL-DCs from WT and Cd36 ^−/− mice were stimulated with CD36-adherent IRBCs for 6 h and then co-cultured with T cells from OT-II transgenic mice in the presence of OVA^323–339 peptide for 72 h. Untreated DCs and DCs stimulated with RBCs, LPS (100 ng/ml) or CpG (2 µg/ml) were used as controls. DCs cultured alone or co-cultured with OT-II T cells and stimulated with IRBCs in absence of OVA^323–339 peptide were also used as controls. IFN-γ secreted into the culture media was analyzed by ELISA. Data are a representative of three independent experiments. Error bars represents mean values ± SD. ***, p<0.001.