Fig. 2.

Gene constructs used to introduce squalene synthase (SQS) and farnesyl diphosphate synthase (FPS) genes into the genome of transgenic plants, and to target the encoded catalytic activities to the cytoplasm or chloroplast (tp) compartments. Promoters: cbt1 cembratrienol synthase (Ennajdaoui et al. 2010), cyp16 cytochrome P450 71D16 (Wang et al. 2002), e duplicated transcriptional enhancer sequence found in the CaMV 35S promoter (Benfey et al. 1990), cv 35S cassava mosaic virus (Verdaguer et al. 1998), ca 35S cauliflower mosaic virus (Benfey and Chua 1990). Genes; ySQS the yeast squalene synthase with a truncation of 168 bp at the 3′ end (Zhang et al. 1993), FPS the chicken farnesyl diphosphate synthase (Tarshis et al. 1994), tp the chloroplast targeting signal sequence from the Arabidopsis Rubisco small subunit gene (Lee et al. 2006)