Table 1. Screen of T₀ transgenic lines for their squalene content.

Construct	# of lines evaluated	Ave (μg/g fw)	Min	Max	Dwarf	Chlorotic	Dwarf and chlorotic
Wild type (control)	15	3.5	0.5	7.4	0	0	0
Trichome SQS only cytosolic	24	7.6	1.0	34.2	1	0	0
Trichome SQS only plastidic	18	6.7	0.6	30.7	0	0	0
Constitutive SQS + FPS cytosolic	29	5.5	0.8	38.7	5	0	0
Trichome SQS +FPS cytosolic	16	8.1	1	20.1	1	0	0
Constitutive SQS + FPS plastidic	26	63.9	1.4	659.7	6	0	0
Trichome SQS + FPS plastidic	17	101.8	3.6	203.5	6	3	5

Transgenic plantlets generated with the indicated construct (expression promoter, gene(s) and intracellular targeting information) were propagated under sterile conditions until they established root systems and were ready for growth in the greenhouse. The greenhouse-grown plants were screened several independent times for their squalene content. In the data shown, plants were from 40 to 60 cm tall and the first fully expanded leaf was sampled. Data represent the average from all the independent plant lines sampled twice, as well as the minimum (min) and maximum (max) observed. Plants were scored as dwarf if their height was 25 % less than their sibling plants. Plants were scored as chlorotic if there was obvious yellowing within 3 or more leaves