Fig. 5.

FTIR spectroscopic analysis of mineral formed in MC3T3 osteoprogenitor cell cultures on RFGD-pretreated or HEAT-pretreated fibronectin-coated or uncoated Ti6Al4V disks. Cells were plated on disks and incubated with cell culture media containing 50 μg/ml ascorbic acid and 3mM β-glycerophosphate for 4 weeks. FTIR spectroscopic analyses were performed on pellets prepared from KBr combined with air-dried samples of the material that covered the disks. Combined spectra obtained from synthetic hydroxyapatite (HYDROXYAPATITE) and (A) RFGD-pretreated fibronectin-coated (RFGD+FN) and uncoated (RFGD NO FN) disks; or (B) HEAT-pretreated fibronectin-coated (HEAT+FN) and uncoated (HEAT NO FN) disks, are shown. PO4—characteristic apatite phosphate peak (900–1,200 cm⁻¹). AMIDE I and AMIDE II— characteristic extracellular matrix protein amide peaks. FN, fibronectin; HEAT, heat pretreatment; RFGD, radiofrequency plasma glow discharge pretreatment.