Table 1.

Comparison of efficacy and reproducibility of influenza A and B extraction on three automated systems.

	TruTip/epMotiona			easyMAGa			QIAcubeb
	Input (gc)	Average Ct ± StDev	% CV	Input (gc)	Average Ct ± StDev	% CV	Input (gc)	Average Ct ± StDev	% CV
Influenza A	7.45 × 106	24.77 ± 0.08	0.33	7.45 × 106	24.01 ± 0.08	0.33	3.73 × 106	24.09 ± 0.26	1.08
	7.45 × 105	28.30 ± 0.49	0.49	7.45 × 105	27.22 ± 0.12	0.44	3.73 × 105	27.41 ± 0.30	1.09
	7.45 × 104	31.92 ± 0.38	0.38	7.45 × 104	30.72 ± 0.19	0.62	3.73 × 104	30.66 ± 0.22	0.72
	7.45 × 103	35.12 ± 0.34	0.97	7.45 × 103	34.74 ± 0.44	1.27	3.73 × 103	34.04 ± 0.23	0.68
	7.45 × 102	38.32 ± 1.15c	3.00	7.45 × 102	36.58 ± 1.07	2.93	3.73 × 102	37.68 ± 0.35	0.93
	7.45 × 101	1/8 detected	NC	7.45 × 101	0/8 detected	NC	3.73 × 101	3/8 detected	NC
Influenza B	3.70 × 106	25.01 ± 0.16	0.64	3.70 × 106	23.44 ± 0.06	0.26	1.85 × 106	23.54 ± 0.39	1.66
	3.70 × 105	27.82 ± 0.28	1.01	3.70 × 105	26.79 ± 0.08	0.30	1.85 × 105	26.98 ± 0.29	1.07
	3.70 × 104	31.21 ± 0.15	0.49	3.70 × 104	30.14 ± 0.17	0.17	1.85 × 104	30.04 ± 0.35	1.17
	3.70 × 103	34.68 ± 0.18	0.52	3.70 × 103	33.52 ± 0.10	0.30	1.85 × 103	33.25 ± 0.46	1.38
	3.70 × 102	38.54± 0.78d	2.02	3.70 × 102	36.71 ± 0.59	1.61	1.85 × 102	36.31 ± 0.30	0.83
	3.70 × 101	1/8 detected	NC	3.70 × 101	3/8 detected	NC	1.85 × 101	4/8 detected	NC

^aValues are the average from 3 or 4 extractions for each dilution (input volume = 250 µL, elution volume = 100 µL) analyzed in duplicate by RT-PCR (n = 6 or 8).

^bValues are the average of 4 extractions per dilution (input volume = 125 µL, elution volume = 50 µL) analyzed in duplicate by RT-PCR (n = 8).Thus the calculated amounts used in equivalent PCR assays are equal in all three methods.

^c6 of 8 RT-PCR replicates detected

^d7 of 8 RT-PCR replicates detected

NC=Not calculated