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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: J Clin Virol. 2013 Jul 21;58(1):10.1016/j.jcv.2013.06.033. doi: 10.1016/j.jcv.2013.06.033

Table 2.

TruTip/epMotion and easyMAG extraction comparison from clinical specimens with two commercially-available real-time one-step RT-PCR master mixes.

qScript™ One-Step qRT-PCR Kit ABI TaqMan® Fast Virus 1step RT-PCR
Master Mix
Number
of
Samples		 easyMAG
Ct range		 Mean
ΔCta 		StDev 95%
confidence
intervalb
(CI)		 Number
of
Samples		 easyMAG
Ct range		 Mean
ΔCta 		StDev 95%
confidence
intervalb
(CI)
4 17.00 – 20.30 1.42 0.92 1.42 ± 0.90 Only 1 sample with Ct < 19.00
30 20.31 – 23.60 1.82 1.02 1.82 ± 0.37 7 19.00 – 23.60 2.04 0.90 2.04 ± 0.67
74 23.61 – 26.90 2.14 0.98 2.14 ± 0.22 19 23.61 – 26.90 1.39 1.04 1.39 ± 0.47
40 26.91 – 30.20 2.40 0.65 2.40 ± 0.20 27 26.91 – 30.20 1.40 1.00 1.40 ± 0.38
12 30.21 – 33.50 1.60 0.86 1.60 ± 0.49 9 30.21 – 33.50 1.04 0.47 1.04 ± 0.31
5 33.51 – 36.80 1.57 1.23 1.57 ± 1.08 3 33.51 – 36.80 0.47 0.58 0.47 ± 0.66
3 36.81 – 40.10 0.97 1.62 0.97 ± 1.83 4 36.81 – 40.10 −0.14 2.05 −0.14 ± 2.01
N = 168 Average = 1.89 1.03 1.89 ± 0.16 N = 69 Average = 1.29 1.09 1.29 ± 0.26
a

Mean ΔCt calculated as the average TruTip/epMotion Ct value minus the average easyMAG Ct value.

b

95% CI values represent MeanΔCt ± CI