Skip to main content
. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: J Clin Virol. 2013 Jul 21;58(1):10.1016/j.jcv.2013.06.033. doi: 10.1016/j.jcv.2013.06.033

Table 3.

TruTip/epMotion and QIAcube extraction comparison from clinical specimens with two commercially-available real-time one-step RT-PCR master mixes.

qScript™ One-Step qRT-PCR Kit ABI TaqMan® Fast Virus 1step RT-PCR
Master Mix
Number
of
Samples		 QIAcube
Ct range		 Mean
ΔCta 		StDev 95%
confidence
interval (CI)b 		Number
of
Samples		 QIAcube
Ct range		 Mean
ΔCta 		StDev 95%
confidence
interval (CI)b
3 17.00 – 20.30 0.98 0.18 0.98 ± 0.20 No samples with Ct < 20.30
29 20.31 – 23.60 1.84 1.03 1.84 ± 0.37 3 20.31 – 23.60 2.42 1.34 2.42 ± 1.52
54 23.61 – 26.90 1.84 1.02 1.84 ± 0.27 12 23.61 – 26.90 1.19 1.27 1.19 ± 0.72
25 26.91 – 30.20 2.00 0.92 2.00 ± 0.36 8 26.91 – 30.20 0.99 0.92 0.99 ± 0.63
6 30.21 – 33.50 2.30 0.61 2.30 ± 0.48 5 30.21 – 33.50 1.24 0.53 1.24 ± 0.46
3 33.51 – 36.80 2.05 1.07 2.05 ± 1.21 3 33.51 – 37.84 0.95 0.15 0.95 ± 0.17
3 36.81 – 40.10 1.28 0.75 1.28 ± 0.85 No samples with Ct > 37.84
N = 123 Average = 1.91 0.96 1.82 ± 0.17 N = 31 Average = 1.24 1.06 1.24 ± 0.37
a

Mean ΔCt calculated as the average TruTip/epMotion Ct value minus the average QIAcube Ct value.

b

95% CI values represent MeanΔCt ± CI