Figure 4.

Western blot analysis of effects of zeaxanthin on expression of Bcl-2 family proteins in uveal melanoma cells. C918 and SP6.5 cell lines were treated with zeaxanthin (30 μM) for 24 h. Cellular protein extracts were subjected to western blot analysis and probed with antibodies specific for proapoptosis member proteins (Bad, Bak, and Bax) and antiapoptosis member proteins (Bcl-2 and Bcl-xL). Calnexin was used as an internal loading control. Representative images are shown at (a). Quantitative results are presented at (b) (SP6.5 cell line) and (c) (C918 cell line). Data are mean ± SD (n = 3). *P < 0.05, versus control (cells cultured without zeaxanthin).