Figure 6.

Effects of zeaxanthin-induced cytochrome c activation on human uveal melanoma cells. Uveal melanoma cells (C918) were treated with zeaxanthin at various doses for 2 h. Cells were collected and lysed, and the cytosol was extracted (see Section 2). The cytochrome c level in the cytosol was measured using a cytochrome c enzyme-linked immunosorbent assay kit. Cytosol cytochrome c level in zeaxanthin-treated cells at different concentrations was expressed as percentage of the controls. Zeaxanthin significantly increased the level of cytosol cytochrome c in a dose-dependent manner. Data are mean ± SD (n = 3). *P < 0.05, versus control (cells cultured without zeaxanthin).