Table 1.
Control | With sulforaphane | Sulforaphane/control | |
---|---|---|---|
Incubated group | 0.0063 ± 0024 | 0.114 ± 0.043 | 18.09 |
Un-incubated group | 0.0020 ± 0.0014 |
Notes: The numbers indicate the rates of DTNB reduction in terms of actual OD410 changes per minute. The results represent means ± SE of six lenses in each incubation group, three lenses in the un-incubated group. The reaction was carried out in a total volume of 200 μL containing 25 μg of the lens proteins, 25 uM NADPH, and 500 μM DTNB. The results were corrected for the reduction rate obtained in the presence of 25 uM aurothiomalate. The reaction was followed for 160 seconds as shown in Figures 1–3. The absorption difference between the sulforaphane and the control group was statistically significant, with a P-value=0.00168.
Abbreviations: DTNB, 5,5′-dithiobis-2-nitrobenzoic acid; NADPH, nicotinamide adenine dinucleotide phosphate (reduced); SE, standard error; OD, optical density.