Table 1.

Absorption changes associated with the reduction of DTNB by thioredoxin reductase activity monitored at 410 nm

	Control	With sulforaphane	Sulforaphane/control
Incubated group	0.0063 ± 0024	0.114 ± 0.043	18.09
Un-incubated group	0.0020 ± 0.0014

Notes: The numbers indicate the rates of DTNB reduction in terms of actual OD410 changes per minute. The results represent means ± SE of six lenses in each incubation group, three lenses in the un-incubated group. The reaction was carried out in a total volume of 200 μL containing 25 μg of the lens proteins, 25 uM NADPH, and 500 μM DTNB. The results were corrected for the reduction rate obtained in the presence of 25 uM aurothiomalate. The reaction was followed for 160 seconds as shown in Figures 1–3. The absorption difference between the sulforaphane and the control group was statistically significant, with a P-value=0.00168.

Abbreviations: DTNB, 5,5′-dithiobis-2-nitrobenzoic acid; NADPH, nicotinamide adenine dinucleotide phosphate (reduced); SE, standard error; OD, optical density.