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Determination of H2O2 working conditions. H9c2 cells were cultured with different concentrations of H2O2 for 1 h (a) or with 100 μM for different incubation times (b). Cell viability was detected by MTT assay. Values are expressed as mean ± SD from three independent experiments. **P < 0.01 versus control; *P < 0.05 versus control.
