Figure 8.

BNC reduced H₂O₂-induced cytosolic Ca²⁺ concentrations in H9c2 cells, as observed by Fluo-3/AM staining with FCM analysis. (a) H9c2 cells without BNC or H₂O₂ (control); (b) H9c2 cells exposed to 100 μM H₂O₂ (model); ((c)–(h)) H9c2 cells pretreated with 0, 7.81, 15.63, 31.25, 62.50 μg/mL BNC, or 10 μM TMZ followed by the treatment with 100 μM H₂O₂. Values are expressed as mean ± SD from three independent experiments. **P < 0.01 versus control; *P < 0.05 versus control; ^## P < 0.01 versus model; ^# P < 0.05 versus model.