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Representation of an OML. Man3-DPPE was prepared by reductive amination of an aldehyde group at the end of the mannotriose (Man3) with an amino group of DPPE. OMLs are prepared from DPPC, cholesterol, and Man3-DPPE at a molar ratio of 10 : 10 : 1 by intense vortex dispersion with antigen-containing PBS and are extruded through a 1 μm pore membrane.
