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. 2013 Oct 29;4:413. doi: 10.3389/fpls.2013.00413

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Copyright © 2013 Müller, Galliardt, Schneider, Barisas and Seidel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of Distance-dependency of ECFP/EYFP and mTurquoise2/mVenus. Based on the R₀-values the FRET-efficiency was plotted against the distance. The graph shows the curves for the FRET-pairs ECFP/EYFP (gray line, source of R₀: Patterson et al., 2000) and mTurquoise2/mVenus (black line, source of R₀: Goedhart et al., 2012). Underneath, the dynamic range is given for both FRET-pairs. The dynamic range corresponds to 0.5 R₀ − 1.5R₀.