Figure 6.

Chlorophyll autofluorescence in Arabidopsis protoplasts. (A) Autofluorescence was detected in the range of 650–700 nm using a 458 nm laser line for excitation by confocal laser scanning microscopy of an Arabidopsis mesophyll protoplast. A z-stack was performed and an average projection is shown. The detected emission is restricted to chloroplasts. (B) Subsequently, emission spectra of mesophyll protoplasts were recorded by CLSM. The minimum of emission at 514 nm was caused by the applied main beam splitter that is designed for simultaneous excitation at 458 and 514 nm. Autofluorescence emission could be observed in a broad wavelength range with a highly dominant peak at 670 nm.