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Activation and inactivation cycles of Dronpa in plant cells. Switching of Dronpa was analyzed by confocal laser scanning microscopy of Arabidopsis protoplasts. Emission was detected between 500 and 600 nm using excitation of 488 nm. Intensive illumination with 488 nm was performed to switch Dronpa off. Subsequent irradiation at 405 nm fully recovered the fluorescent state. The procedure was repeated for 10 times without significant loss in emission intensity.
