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. 2013 Oct 29;4:413. doi: 10.3389/fpls.2013.00413

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Copyright © 2013 Müller, Galliardt, Schneider, Barisas and Seidel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Donor-quenching of Dronpa in the presence of mCherry. The comparison of emission spectra of Dronpa and the fusion protein Dronpa-mCherry revealed the quenching of Dronpa in the presence of mCherry (A). In the emission spectrum of Dronpa-mCherry the emission of mCherry is less pronounced due to the low quantum yield of mCherry. The same effect can be observed by the emission of eluted fluorescent proteins (B): whereas mCherry showed red fluorescence, the emission of the fusion protein is dominated by the yellowish Dronpa fluorescence.