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Influence of photoswitching on FRET-ratio of a Dronpa-mCherry fusion. Ratio imaging was performed as a time series. Images were obtained every 6–8 s for 10 min. (A) Dronpa was inactivated during the time series. (B) Dronpa was reactivated at the time points by irradiation with a 405 nm diode laser. The black line shows data that has not been corrected for acceptor spectral bleed through while the gray curve shows the corrected data.
