Table 3.
Förster-radii of fluorescent protein FRET-pairs.
| Fluorophores | Förster radius R0 | Dynamic range | References |
|---|---|---|---|
| BLUE DONOR | |||
| EBFP/ECFP | 3.8 nm | 1.9–5.7 nm | Patterson et al., 2000 |
| EBFP/EGFP | 4.1 nm | 2.1–6.2 nm | Patterson et al., 2000 |
| EBFP/EYFP | 3.8 nm | 1.9–5.7 nm | Patterson et al., 2000 |
| EBFP/DsRed | 3.2 nm | 1.6–4.8 nm | Patterson et al., 2000 |
| ECFP/EGFP | 4.8 nm | 2.4–7.2 nm | Patterson et al., 2000 |
| ECFP/EYFP | 4.9 nm | 2.5–7.3 nm | Patterson et al., 2000 |
| ECFP/mVenus | 5.0 nm | 2.5–7.5 nm | Rizzo et al., 2006 |
| mCerulean/EYFP | 5.4 nm | 2.7–8.1 nm | Rizzo et al., 2006 |
| mCerulean/mVenus | 5.4 (5.2) nm | 2.7–8.1 nm | Rizzo et al., 2006; Markwardt et al., 2011 |
| mCerulean/mCitrine | 5.4 nm | 2.7–8.1 nm | Rizzo et al., 2006 |
| mCerulean3/mVenus | 5.7 nm | 2.9–8.6 nm | Markwardt et al., 2011 |
| SCFP3/SYFP2 | 5.4 nm | 2.7–8.1 nm | Goedhart et al., 2007 |
| mTurquoise/mVenus | 5.7 nm | 2.9–8.6 nm | Markwardt et al., 2011 |
| mTurquoise2/mVenus | 5.8 nm | 2.9–8.7 nm | Goedhart et al., 2012 |
| ECFP/DsRed | 4.2 (5.1) nm | 2.1–6.3 nm (2.6–7.7 nm) | Patterson et al., 2000; Erickson et al., 2003 |
| ECFP/mCherry | 3.5 nm | 1.8–5.3 nm | He et al., 2005 |
| EGFP/EYFP | 5.6 nm | 2.8–8.4 nm | Patterson et al., 2000 |
| EGFP/DsRed | 4.7 (5.8) nm | 2.4–7.1 nm (2.9–8.7 nm) | Erickson et al., 2003 |
| EGFP/mRFP1 | 4.7 nm | 2.4–7.1 nm | Peter et al., 2005 |
| Clover/mRuby2 | 6.3 nm | 3.2–9.5 nm | Lam et al., 2012 |
| Kaede/Kaede | 5.8 nm | 2.9–8.7 nm | Wolf et al., 2013a |
| Dronpa/mCherry | 5.6 nm | 2.8–8.4 nm | This work |
| EYFP/DsRed | 4.9 nm | 2.5–7.4 nm | Patterson et al., 2000 |
| EYFP/mCherry | 5.7 nm | 2.9–8.6 nm | Akrap et al., 2010 |
| SYFP2/mStrawberry | 6.3 nm | 3.2–9.5 nm | Goedhart et al., 2007 |
| mKo/mCherry | 6.4 nm | 3.2–9.6 nm | Goedhart et al., 2007 |