Table 1. Comparison of biodosimetry method properties.
Method | Dose Range (Gy) |
Time before sample can be taken |
Time to dose estimate |
Time period when assay will give results |
---|---|---|---|---|
Dicentric Chromosome | 0.1–5 | Immediately | 55 + h | 3–6+ mo |
Assay | 0.5–5 (triage) | 52+ h | ||
Cytokinesis Block
Micronucleus |
0.3–5 | Immediately | 3+ d | 6 mo –1 y |
γ–H2AX | Immediately | 24–48 h | ||
Microscope | 0.5–5 | 4–6 h | ||
FACS | 0.5–10 | 2 h | ||
Lymphocyte Depletion
Kinetics Assay |
0.5/1–10 | Immediately for baseline, time needed for depletion to occur |
<1 h per test. Serial testing requires 8-48 h. |
12 h– 7 d |
Premature Chromosome Condensation | 0.2–20 | Immediately | CHO fusion: 2–3 h Chemical stimulation: 51 h |
At least 1 wk, likely 6+ mo |
Gene Expression | 0.1–5 | Immediately, depending on when gene is upregulated |
9–36 h | Through 48–72 h |
EPR (tooth) | 1–30 | Immediately | 5–25 min | Lifetime and longer |