Figure 3.

(A) Effects of i.c.v. injections of modified teleost Ringer's (30% methanol, 70% teleost Ringer's) or serotonin (5-HT) alone, or in combination with the mixed 5-HT₁/5-HT₂ receptor antagonist, methysergide on food intake in rainbow trout. Assessment of food intake was carried out as in Figure 1. Bars that do not share a common letter are significantly different from each other as determined by a One-Way ANOVA and by a pairwise Tukey's test (P < 0.05). Values are means + sem (n = 8). (B) Hill plot demonstrating the half-maximal effective dose (ED₅₀) of 5-HT (ED₅₀ = 14.7 ng/g BW) i.c.v. injections on food intake (FI).