Table 1.

Viral constructs for specific targeting mouse astrocytes.

Viral constructa	Applicationsb	Brain regions	Specificity	References
AAV1/2-GFAP-GFP AAV1/2-CBA-GFPc	IC at P0, P90	Cortical and subcortical	AAV1/2-GFAP-GFP expressed mostly ALDH1L1(+) cells, AAV1/2-CBA-GFP specific for subcortical neurons at P90	von Jonquieres et al., 2013
AAV2/5-gfaABC1D-Lck-GCaMP3d AAV2/5-gfaABC1D-GCaMP3	IC at P49 to P63	CA1 region	Labels GFAP(+) cells	Shigetomi et al., 2013b
AAV8-CBAe-eYFP-2A-tTA(2S)	IVE at P3	Whole brain	Labels S100β (+) cells	Kim et al., 2013
AAV2/5-, AAV2/8-, AAV2/9-CBA-EGFP	IVE at P0 to P3	Whole brain	AAV2/5 at P0 to P3 transduces mostly GFAP(+) cells, AAV2/8, AAV2/9 at P3 transduce mostly GFAP(+) cells	Chakrabarty et al., 2013
AV-CMV-Flox-ChR2-mCherry (injected in hGFAP-Cre line)	IC at P14	CA1 region	Labels GFAP(+) cells, but not NeuN(+) cells	Chen et al., 2013a
AAV1-, AAV8-, AAV9, AAVrh10-CAG-GFP	IV at P0, P5, P14, P42	Whole brain	Both neurons and astrocytes were labeled	Miyake et al., 2011
scAAV7-, AAV9-, rh10-rh39-, rh43-CB-EGFPf	IV at P1	Whole brain	Both neurons and astrocytes were labeled	Zhang et al., 2011
AV-mCMV-gfaABC1D-Case12 AV-mCMV-gfaABC1D-ChR2(H134R)-Katushka1.3	IC	Hypoglossal motor nucleus	All labeled cells are GFAP(+)	Gourine et al., 2010
AAV2/5-, AAV2/9-CMV-EGFP AAV2/5-gfa104-EGFP	IC	CA1 region	AAV5-CMV shows tropism for astrocytes	Ortinski et al., 2010
			AAV5-gfa104 >99% selectivity for astrocytes
AAV2/5-gfaABC1D-mRFP-p130PH	IC	Cortex Hippocampus	Selective expression in GFAP(+) cells	Xie et al., 2010
LV-GFAP-hChR2(H134R)-mCherry	IC	Subthalamic nucleus	Labelled cells are GFAP(+)	Gradinaru et al., 2009
LV-PGK-nlsLacZ -miR124T/ LV-PGK-GLAST-miR124T Mokola pseudotyped	IC	Hippocampus, Cerbellum, Striatum	6% neuronal expression in striatum	Colin et al., 2009
scAAV9-CB-GFP	IV, tail injection at P70	Whole brain	Labels mostly astrocytes	Foust et al., 2009
LV-mCMV-gfaABC1D-EGFP	IC	Hypoglossal motor nucleus	All labeled cells are GFAP positive	Liu et al., 2008

^aWe keep the names given in the quoted papers. Notation AAV2/x (also called AAVx) stands for pseudotyped AAV. ITR of coding plasmid derived from AAV serotype 2 and capsid sequence from serotype x.

^bIC, intracerebral; IV, intravenous; IVE, intraventricular; P0 day of birth.

^cAAV1/2 chimeric AAV with capsid proteins from AAV1 and AAV2.

^dgfaABC₁D (also called gfa104) is a short (681 bp) version of the hGFAP promoter.

^eCBA, CB, CAG, hybrid promoter composed of CMV early enhancer element and chicken β-actin promoter.

^fSc, self complementary, see