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Results of calorimetric screening of PYP deletion mutants. The color change of 12 deletion mutants upon adding a precursor of chromophore is shown. Persistent color change was observed for N5, N10, and N18, but the rest of the N-terminal deletion mutants and all C-terminal deletion mutants showed weak color change that gradually faded. The N27C18 deletion mutant can be still used to check expression of the target protein as it shows color change for a few minutes.
