Table 1. Primer sequences of 24 additional microsatellite and minisatellite loci used in the multilocus typing analysis of Cryptosporidium hominis*.
| No. | Locus | Polymorphism | Primer | Sequence, 5′→3′ | PCR product , bp |
|---|---|---|---|---|---|
| 1 | C6–60 | Microsatellite | F1 | TGGACAAGAGTGTCGCTTCTA | ≈866 |
| CAC | R1 | GTATTGGGTCCTTTCAGCTCA | |||
| F2 | TCCAAAGTACACTGCGTAAATAT | ||||
| R2 | TCATGTCTCAACATGCTGAGTA | ||||
| 2 | C6–160 | Microsatellite | F1 | GTACTCAAGTTCCAGTTGTGGA | ≈582 |
| TA | R1 | CCAATACATTCAGAAGACAGTCT | |||
| F2 | CATCCTCAGAAGAGTACTGTA | ||||
| R2 | GTGCTGAATGGCTTCCTATTA | ||||
| 3 | C6–190 | Microsatellite- | F1 | CTATCTCTCAAGCAATGCAAAC | ≈534 |
| AAT | R1 | CAAGCTCCATCAGATTATCAATA | |||
| F2 | CAAGTACTGGAATAGTTACTCA | ||||
| R2 | CTGGCAATACGTAATGCCTTT | ||||
| 4 | C6–230 | Microsatellite | F1 | GGCCACTTCATGGTAATACTC | ≈627 |
| TAA | R1 | GTCAACGAATCATCCAGAGATT | |||
| F2 | GGTACAATTGGAGGTATCAGT | ||||
| R2 | GAATCTCGAACCTGAAGACTT | ||||
| 5 | C6–280 | Microsatellite | F1 | GATTAAAATCAGGCGGGCCAA | ≈764 |
| TCA | R1 | GAGCAATTTCTTGAATAATATTGAG | |||
| F2 | GAGGACTGTGGGGGAATATAA | ||||
| R2 | GACAGGAAATCCAATCAATGAATTT | ||||
| 6 | C6–350 | Microsatellite | F1 | GTTCTTTCAATTGTGTACCATGA | ≈715 |
| ATT | R1 | GCTATTGGTTCAAATAATGGTATGA | |||
| F2 | GCATCGCAAATCATCATCTATTT | ||||
| R2 | CTACATGGGATTGTTTATATGATA | ||||
| 7 | C6–500′ | Microsatellite | F1 | GACAAAGAGTATTAGCATCGACA | ≈484 |
| ATA | R1 | CTTCATGCTCTAGTTCTTCTAAT | |||
| F2 | GAGTCATATGAAACAGAATCTTT | ||||
| R2 | CAAATTGATCGAGATTTGTTGAA | ||||
| 8 | C6–580 | Microsatellite | F1 | CTGGATGACCAAGGTGTTAAT | ≈632 |
| TCT | R1 | CTGTATTGTTACTGTCAATGCTT | |||
| F2 | GAGATGAATATTGAGAATGAATCCA | ||||
| R2 | CTCGAGATGATCGATGAATCAT | ||||
| 9 | C6–740 | Microsatellite | F1 | GAAACAGTTAAAGATAGCTTGGA | ≈703 |
| AT | R1 | GAAGATGATGGAATTCTTCTCAT | |||
| F2 | GTATCTTATGTGTATTCAAGCAT | ||||
| R2 | CTAATTCTTTGTATGACAAGATCAT | ||||
| 10 | C6–830′ | Microsatellite | F1 | GAGGCCTTAAAGCTTCTTTTA | ≈679 |
| ATT | R1 | GCACTACAACAAGTGGTACTA | |||
| F2 | GAGATTGTATTCTTCTATACCACT | ||||
| R2 | CAAGTTCCAATGATAGGATCAGT | ||||
| 11 | C6–830 | Microsatellite | F1 | GATGTATTACTACCAGAATTGAGA | ≈692 |
| TAG | R1 | CTACTCAATCAATGGAAGTTACT | |||
| F2 | CCAACTGATGATATTCCAGTATT | ||||
| R2 | GGTATTATTACATCTAGTGGTGA | ||||
| 12 | C6–870 | Microsatellite | F1 | CAGTGACCGAATTTACTCTCT | ≈801 |
| AT | R1 | GTCTCTTTAGTCAAGTTTTCCT | |||
| F2 | CGCTCTTCCACGTACAATTT | ||||
| R2 | CGAGAATAAAGCTGACATTTCTT | ||||
| 13 | C6–1000′ | Minisatellite | F1 | GGAGCCTAATTGTGCTCATAT | ≈583 |
| GATAAAAAG | R1 | GGTTATCATGCACTGACTGTA | |||
| AAGAGGGA | F2 | CTCAATTGCTAAAGATGCAGAT | |||
| R2 | GAATCCTGTTGTTCTCCATCT | ||||
| 14 | C6–1420 | Minisatellite | F1 | GCATTAGAGCATCCATGGTTA | ≈534 |
| GAAAGAGA | R1 | CCTGTGGTCTCGATATTCATT | |||
| GCGT | F2 | CATCTTCAGCCGTTGATGAAA | |||
| R2 | GTAAACTCCTTGGAGGTGAAT | ||||
| 15 | C6–2600 | Microsatellite | F1 | GAGGATGAACTTGTACTGCAAT | ≈806 |
| GCAGCT | R1 | CCTAGTATTGGTGGTACTTGTA | |||
| F2 | GGATCGAATTCTGGGTTGAAT | ||||
| R2 | GTATCAACTTGCCCTGGAATA | ||||
| 16 | C6–2970 | Microsatellite | F1 | GACTGTATGCCTTTGTTCCTT | ≈723 |
| GA | R1 | GTTGAAGATACTCCTGAAGCT | |||
| F2 | GAGCGTCTTGTCTACTGACTT | ||||
| R2 | CTGTCCTCCAGGCATTATTGA | ||||
| 17 | C6–3110′ | Minisatellite | F1 | GTGGATAAGAGAACCCGTCAT | ≈725 |
| TTCCTCCTA | R1 | CACAAATTCTGGTGGGAATAGT | |||
| F2 | CCAGTGATAGTTAATCCAGCT | ||||
| R2 | GAGCCAGAAGTCAGTATTTCT | ||||
| 18 | C6–3520 | Microsatellite | F1 | GTTGGAGTATGACAATTACCTAA | ≈748 |
| TTA | R1 | CATCATGGAAATCTTACAGGATT | |||
| F2 | GCATTGTACAACATCCATGTCT | ||||
| R2 | GGTGAACATTTTGGATTAGTATCT | ||||
| 19 | C6–3520′ | Microsatellite | F1 | CTCTGTACAGCTGGTGTAATT | ≈577 |
| TAT | R1 | CCTGGTGTGAATCAATGTTGT | |||
| F2 | CTTCTGGAAGCTCACCAAGAA | ||||
| R2 | GCTCCTTATAGATTAGCTGAGT | ||||
| 20 | C6–3690 | Microsatellite | F1 | CTTCTGGAGATTCCATATCATTGA | ≈640 |
| TCT | R1 | CCTTAAGGCTGGTTGATATGATT | |||
| F2 | GTGCAAGTGATATTTCCACTATCT | ||||
| R2 | CTGTCGAATCACTAGGCAGAA | ||||
| 21 | C6–4110 | Microsatellite | F1 | CGGAAGATAATGCTCAACTGT | ≈709 |
| ACA | R1 | CTGCCTTACTATCATTCGCAT | |||
| F2 | CAAGTGGAAACATTGAGCCAA | ||||
| R2 | GGAACTGACTGGTTTGATGAT | ||||
| 22 | C6–5110′ | Microsatellite | F1 | CACTCTTAATTCCTTCATGGCT | ≈533 |
| CTC | R1 | GGTCAATAGGACGTATTTCTTA | |||
| F2 | GCATCCTCAGCTTTAGAATTCT | ||||
| R2 | GGTACAGAAGATCAGCTTTCT | ||||
| 23 | C6–5120 | Microsatellite | F1 | GGATTGATCAGTGACAGTGAA | ≈569 |
| AT | R1 | CATAATTGGAGGACTATGTCAAAT | |||
| F2 | GCTGTAGTTCTAAGAGTCCAT | ||||
| R2 | GTCTCAAATATCATTAGAACAAGGT | ||||
| 24 | C6–5410 | Microsatellite | F1 | GACTCAGTTCGAGAGAAGTCA | ≈646 |
| GTAGTG | R1 | GGTGAATCATCTTCATGCAGT | |||
| F2 | CATCGAGCGTGCACTTAATCA | ||||
| R2 | CTCAGACTCAAGGACCTGAAT |
*All annealing temperatures for primers were 55°C. F, forward; R, reverse.