Table 1.
Cyst counts in T. gondii infected mice
| Mouse | UIC* | CIM | CIM | CIM | CIM | CIM | CIM | CIM | NMRI | NMRI | NMRI | NMRI |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| T. gondii (# injected) | – | GT-1 500 | GT-1 1000 | BK 5000 | BK 10,000 | NED 10,000 | NED 10,000 | NED 10,000 | GT-1 500 | BK 500 | NED 10,000 | NED 10,000 |
| Q-PCR (cycle) | >35 | 22.9 | 24.2 | 18.7 | 22.0 | 26.9 | 26.2 | 28.8 | Dead | Dead | 22.6 | 20.3 |
| Cysts per brain | 0 | 100 | 50 | 130 | 150 | 220 | 90 | 15 | 720 | 4800 | ||
| Antibody test | – | + | + | + | + | + | + | + | + | + |
*
Uninfected control.
Mice were sacrificed 5 weeks (NED) or 6–8 weeks (BK and GT-1) after tachyzoite injection. Infection was verified by serum antibody. Cysts were evaluated by direct counting in homogenised brains and by quantitative PCR of a repeat element of T. gondii (Reischl et al., 2003) in genomic DNA samples isolated from mouse brains.