Figure 5. Carbonic anhydrase activity and HCO₃⁻ permeability in native and YPet-mAE1-expressing tsA201 cells.

A, intracellular CA activities measured in lysates of untreated tsA201 cells (control) and tsA201 cells transfected with YPet-mAE1 (AE1), using the mass spectrometric ¹⁸O technique. B, cellular measured in untreated tsA201 cells (control) and in tsA201 cells expressing YPet-mAE1 (AE1), in the absence or presence of DIDS (10⁻⁵ m), a strong inhibitor of AE1 transport function. DIDS showed no effect on of control cells, but inhibited the AE1-associated increase in . ns, P > 0.05, *P < 0.02, ^§P < 0.02. Number of measurements are inside the columns. Error bars represent SD values.