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. 2013 Aug 19;591(Pt 20):4983–5004. doi: 10.1113/jphysiol.2013.258673

Figure 2. 5′-RACE analysis of human SLC4A7 transcripts.

Figure 2

5′-RACE was performed with cDNA libraries (Marathon-Ready cDNA, Clontech) of human brain, heart, kidney, liver and skeletal muscle. The PCR products were analysed by agarose gel electrophoresis. A major product of ∼0.3 kb (lower arrow) was obtained for all cases. The entire PCR product was cloned into TOPO vector. Multiple random clones (12 for brain, 8 for kidney, 4 for heart, 6 for skeletal muscle and 9 for liver) were sequenced for each organ. Three different types of transcripts were identified. The first includes exons 1 + 3 + 4 encoding ‘MEAD’ (represented by lower arrow). The second includes exons 2 + 3 + 4 encoding ‘MERF’ (also represented by lower arrow). The third type (from liver) initiates from intron 2–3 and may represent a novel transcript (the ∼1.3 kb band represented by the higher arrow) that we did not further pursue in the present study.