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A, eNOS (Ser635) phosphorylation; B, eNOS (Ser1177) phosphorylation; C, intracellular cAMP contents. Insulin (Ins, 100 nm) and forskolin (F, 10 μm) were used as control. D, endothelial 125I-insulin uptake. Insulin (Ins, 2 μm) was used as positive control, R:3 μM ranolazine. n= 4–8. *P < 0.05 vs. control.
